Bioactivity
Functional readouts on particle-count-matched doses
Every bioactivity assay is dosed on particle count, not protein mass. We routinely include synthetic-particle controls (custom liposomes) at matched count so the "EV effect" is separable from a general nanoparticle effect.
Macrophage activation (THP-1)
NF-κB reporter and cytokine readout in THP-1 macrophages. Standard readout for pro-inflammatory / anti-inflammatory EV phenotypes.
Scratch / wound-healing
2D migration and closure assays for regenerative and oncology EV preparations.
HUVEC tube formation
Endothelial tube formation as an angiogenesis readout - relevant for tumor- and stem-cell-derived EV programs.
DC maturation
Dendritic cell maturation marker panels by flow cytometry for immuno-oncology and vaccine-relevant EV work.
Phospho-flow signaling
Pathway-specific phospho-flow readouts in recipient cells to dissect intracellular signaling responses to EV uptake.
Custom assay development
Cell-line-specific or pathway-specific assays designed against your scientific question. Discovery-tier engagements.